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chemically synthesized as gene fragments  (Twist Bioscience)


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    Twist Bioscience chemically synthesized as gene fragments
    Chemically Synthesized As Gene Fragments, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chemically+synthesized+as+gene+fragments/gene+fragments/pm36687471-62-42-44
    Average 90 stars, based on 1 article reviews
    chemically synthesized as gene fragments - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference.
    Article Snippet: Oligonucleotides were synthesized by Integrated DNA Technologies. .. The bgaL promoter and the bgaR gene encoding the regulator, dFnCas12a gene and FnCas12a scaffold were cloned into the pMTL85141 [36] shuttle vector to produce pJRJ001. dFnCas12a was codon optimized for expression in Clostridium (IDT codon optimization tool) and chemically synthesized as gene fragments (Twist Bioscience). .. The bgaL promoter and bgaR gene were amplified from the pKOD_mazF plasmid [13] using primers 138 and 139 and the FnCas12a scaffold, directly followed by a lacZα gene, was synthesized as fragments (gBlocks, IDT) and PCR amplified using primers 144 and 171.

    Expressing:

    Article Title: Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference.
    Article Snippet: Oligonucleotides were synthesized by Integrated DNA Technologies. .. The bgaL promoter and the bgaR gene encoding the regulator, dFnCas12a gene and FnCas12a scaffold were cloned into the pMTL85141 [36] shuttle vector to produce pJRJ001. dFnCas12a was codon optimized for expression in Clostridium (IDT codon optimization tool) and chemically synthesized as gene fragments (Twist Bioscience). .. The bgaL promoter and bgaR gene were amplified from the pKOD_mazF plasmid [13] using primers 138 and 139 and the FnCas12a scaffold, directly followed by a lacZα gene, was synthesized as fragments (gBlocks, IDT) and PCR amplified using primers 144 and 171.

    Synthesized:

    Article Title: Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference.
    Article Snippet: Oligonucleotides were synthesized by Integrated DNA Technologies. .. The bgaL promoter and the bgaR gene encoding the regulator, dFnCas12a gene and FnCas12a scaffold were cloned into the pMTL85141 [36] shuttle vector to produce pJRJ001. dFnCas12a was codon optimized for expression in Clostridium (IDT codon optimization tool) and chemically synthesized as gene fragments (Twist Bioscience). .. The bgaL promoter and bgaR gene were amplified from the pKOD_mazF plasmid [13] using primers 138 and 139 and the FnCas12a scaffold, directly followed by a lacZα gene, was synthesized as fragments (gBlocks, IDT) and PCR amplified using primers 144 and 171.



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    ( A ) Immunoblots of sgCtrl and PDAP1-KO1.4 cell lysates collected 12 hours after tunicamycin (Tm) treatment. ( B ) Dicistronic HAV IRES reporter activity in tunicamycin-treated Huh-7.5 cells. Cells were transfected with plasmid DNA 1 hour after addition of tunicamycin and harvested 24 hours later for FLuc and RLuc assays. N = 3 technical replicates from a representative experiment. P values by one-way ANOVA. LU, light units; AU, arbitrary units. ( C ) NLuc activity reflecting replication of the CHIKV-NLuc reporter virus in sgCtrl and PDAP1-KO1.4 cells 24 hours after infection. MOI, multiplicity of infection. ( D ) Immunoblots of CHIKV capsid protein, p-eIF2a, and eIF2a in lysates from sgCtrl and PDAP1-KO1.4 cells 24 hours after CHIKV-NLuc infection. ( E ) Dicistronic HAV IRES reporter [see (B)] activity in CHIKV-NLuc–infected sgCtrl cells. Cells were transfected with plasmid DNA 1 hour after infection and harvested 24 hours later for FLuc and RLuc assays. P values by one-way ANOVA. Data shown represent N = 3 technical replicates. ( F ) Immunoblots of GFP expressed by circRNA IRES reporters containing BiP, c-Myc, and XIAP IRES sequences in tunicamycin-treated sgCtrl versus PDAP1-KO1.4 cells. ( G ) BiP, c-Myc, and XIAP translational efficiencies in sgCtrl and PDAP1-KO1.4 cells, with and without tunicamycin treatment, based on GFP expression normalized to circRNA abundance measured by RT-qPCR. P values by two-way ANOVA, with corrections for multiple comparisons using the Benjamini, Krieger, and Yekutieli method. N = 3 independent experiments.

    Journal: Science Advances

    Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses

    doi: 10.1126/sciadv.adq6342

    Figure Lengend Snippet: ( A ) Immunoblots of sgCtrl and PDAP1-KO1.4 cell lysates collected 12 hours after tunicamycin (Tm) treatment. ( B ) Dicistronic HAV IRES reporter activity in tunicamycin-treated Huh-7.5 cells. Cells were transfected with plasmid DNA 1 hour after addition of tunicamycin and harvested 24 hours later for FLuc and RLuc assays. N = 3 technical replicates from a representative experiment. P values by one-way ANOVA. LU, light units; AU, arbitrary units. ( C ) NLuc activity reflecting replication of the CHIKV-NLuc reporter virus in sgCtrl and PDAP1-KO1.4 cells 24 hours after infection. MOI, multiplicity of infection. ( D ) Immunoblots of CHIKV capsid protein, p-eIF2a, and eIF2a in lysates from sgCtrl and PDAP1-KO1.4 cells 24 hours after CHIKV-NLuc infection. ( E ) Dicistronic HAV IRES reporter [see (B)] activity in CHIKV-NLuc–infected sgCtrl cells. Cells were transfected with plasmid DNA 1 hour after infection and harvested 24 hours later for FLuc and RLuc assays. P values by one-way ANOVA. Data shown represent N = 3 technical replicates. ( F ) Immunoblots of GFP expressed by circRNA IRES reporters containing BiP, c-Myc, and XIAP IRES sequences in tunicamycin-treated sgCtrl versus PDAP1-KO1.4 cells. ( G ) BiP, c-Myc, and XIAP translational efficiencies in sgCtrl and PDAP1-KO1.4 cells, with and without tunicamycin treatment, based on GFP expression normalized to circRNA abundance measured by RT-qPCR. P values by two-way ANOVA, with corrections for multiple comparisons using the Benjamini, Krieger, and Yekutieli method. N = 3 independent experiments.

    Article Snippet: Infectious molecular HAV clones pHAV/p16.2 , pHAV/18f.2 , p18f-NLuc and p18f-NLuc/GAA , p18f-FLuc and p18f-FLuc/GAA replicon clones , pRV-B14-NLuc , pPV-1/FLuc ( ) replicons, and bicistronic HAV IRES reporter pFL-HAV-RL ( ) have been described previously. pPDAP1-Flag, encoding C-terminal Myc/Flag-tagged human PDAP1 (NM_014891.7) under the control of the cytomegalovirus promoter, was purchased from Origene (pRC20058). pPDAP1-Y124 was generated from pPDAP1-Flag by PCR mutagenesis. pMCSG9-PDAP1-Avi, a bacterial expression vector encoding maltose-binding protein fused to PDAP1 with an intervening tobacco etch virus cleavage sequence, was constructed by inserting the PDAP1 sequence from pPDAP1-Flag into pMCSG9 ( ); a C-terminal GLNDIFEAQKIEWHE Avi tag was added by PCR-based mutagenesis. circRNA reporter plasmids for the EMCV (TR-circGFP), KSHV vFLIP, and PV IRES have been described previously ( , ). circRNA reporter plasmids containing IRES elements existing in transcripts encoding BiP (HSPA5, GRP78) (NM_005347.5, nucleotides 1 to 220) , XIAP (NG_007264.1, nucleotides 30293 to 30468) , and c-Myc (NM_001354870.1, nucleotides 798 to 1205) ( ) IRES elements were constructed by Gibson assembly using chemically synthesized DNA gene fragments (Genewiz, Azenta).

    Techniques: Western Blot, Activity Assay, Transfection, Plasmid Preparation, Virus, Infection, Expressing, Quantitative RT-PCR